q10 array proteinchip seldi tof analysis  (Bio-Rad)

Journal: PLoS ONE

Article Title: A Novel Truncated Form of Serum Amyloid A in Kawasaki Disease

doi: 10.1371/journal.pone.0157024

Figure Lengend Snippet: The peak is shown at mass = 7,860 Da on CM10 ProteinChip arrays (A) in the pH 9 fraction after application of KD and FC plasma to Q anion exchange ceramic beads at pH 9; (B) in 50% ACN reverse phase fraction after application of the KD sample from (A) to PLRP beads, (C) the KD sample after using an ultrafiltration centrifugation device, and (D) the KD sample after elution from SDS-PAGE band. The FC spectrum is shown only for (A).

Article Snippet: Aliquots of each fraction (10 μL) were added to 90 μL binding buffer and applied to ProteinChip SELDI arrays (Bio-Rad Laboratories, Hercules, CA).

Techniques: Centrifugation, SDS Page

Journal: International Journal of Proteomics

Article Title: The Human Urinary Proteome Fingerprint Database UPdb

doi: 10.1155/2013/760208

Figure Lengend Snippet: Overview of SELDI-TOF spectra available as part of the database. Spectra were recorded using low laser energy desorption ( m/z range 1500 to 25000) and high laser energy desorption ( m/z range 20000 to 150000) from 200 nonredundant samples. Peak clusters common in all spectra of one chip-type were analysed using a 5% signal-to-noise cutoff, and numbers of peaks found in at least 10% or 20% of all spectra were counted.

Article Snippet: 0.1 mL human urine was applied directly to preconditioned SELDI ProteinChip arrays (Bio-Rad Laboratories Inc.) (NP20, H50, SEND, Q10, CM10 and IMAC30), as recommended by the manufacturer, in a ProteinChip bioprocessor and incubated with 0.1 mL binding buffer where appropriate.

Techniques: Binding Assay

Journal: International Journal of Proteomics

Article Title: The Human Urinary Proteome Fingerprint Database UPdb

doi: 10.1155/2013/760208

Figure Lengend Snippet: Example of SELDI mass spectra of human urine using various chip surfaces. 0.1 mL urine from a healthy control sample (a) and from a cancer patient (b) was applied to the chip surfaces, as recommended by the manufacturer and analysed by SELDI-TOF. The spectra are plotted as m/z (6000 to 13500) against intensity.

Article Snippet: 0.1 mL human urine was applied directly to preconditioned SELDI ProteinChip arrays (Bio-Rad Laboratories Inc.) (NP20, H50, SEND, Q10, CM10 and IMAC30), as recommended by the manufacturer, in a ProteinChip bioprocessor and incubated with 0.1 mL binding buffer where appropriate.

Techniques: Control

Journal: International Journal of Proteomics

Article Title: The Human Urinary Proteome Fingerprint Database UPdb

doi: 10.1155/2013/760208

Figure Lengend Snippet: Current number of entries in UPdb by source. All current entries in the UPdb database were tallied based on the MS technique used. The number of detected urinary peaks together with the covered mass range, the median m/z , the disease areas studied, the number of identified proteins, and the number of datasets retrieved from the literature are listed.

Article Snippet: 0.1 mL human urine was applied directly to preconditioned SELDI ProteinChip arrays (Bio-Rad Laboratories Inc.) (NP20, H50, SEND, Q10, CM10 and IMAC30), as recommended by the manufacturer, in a ProteinChip bioprocessor and incubated with 0.1 mL binding buffer where appropriate.

Techniques: